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cd227 pe vio770  (Miltenyi Biotec)


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    Miltenyi Biotec cd227 pe vio770
    The amount of urinary TECs can be measured using flow cytometry. (a) Gating strategy for selecting cytokeratin positive single cells. Staining of CD10, CD13, CD326, and (b) <t>CD227</t> compared with isotype-controls in postmortem kidney tissue, (c) postmortem ureteral tissue, and (d) urine. Note CD326 but not CD227 positivity in ureteral epithelial cells. (e,f) Fluorescence microscopic image of (e) purified CK+DAPI+CD13+CD10+ cells, and (f) purified CK+DAPI+CD326+CD227+ cells (blue = DAPI, green = cytokeratin). (g–j) Electron microscopic images of purified cytokeratin+CD227+ cells using a cell sorter. (g and detail h) Apoptotic cell with characteristic marginalization of condensed chromatin (yellow arrows in h) and intact plasma membrane. (i and j) Necrotic cells with chromatin clumping (red arrow in j) and loss of plasma membrane integrity as distinguishing feature from apoptosis (yellow arrow in j). TECs, tubular epithelial cells.
    Cd227 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd227+pe/pmc12034873-166-43-51?v=Miltenyi+Biotec
    Average 93 stars, based on 5 article reviews
    cd227 pe vio770 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Flow-Cytometric Quantification of Urine Kidney Epithelial Cells Specifically Reflects Tubular Damage in Acute Kidney Diseases"

    Article Title: Flow-Cytometric Quantification of Urine Kidney Epithelial Cells Specifically Reflects Tubular Damage in Acute Kidney Diseases

    Journal: Kidney International Reports

    doi: 10.1016/j.ekir.2025.01.037

    The amount of urinary TECs can be measured using flow cytometry. (a) Gating strategy for selecting cytokeratin positive single cells. Staining of CD10, CD13, CD326, and (b) CD227 compared with isotype-controls in postmortem kidney tissue, (c) postmortem ureteral tissue, and (d) urine. Note CD326 but not CD227 positivity in ureteral epithelial cells. (e,f) Fluorescence microscopic image of (e) purified CK+DAPI+CD13+CD10+ cells, and (f) purified CK+DAPI+CD326+CD227+ cells (blue = DAPI, green = cytokeratin). (g–j) Electron microscopic images of purified cytokeratin+CD227+ cells using a cell sorter. (g and detail h) Apoptotic cell with characteristic marginalization of condensed chromatin (yellow arrows in h) and intact plasma membrane. (i and j) Necrotic cells with chromatin clumping (red arrow in j) and loss of plasma membrane integrity as distinguishing feature from apoptosis (yellow arrow in j). TECs, tubular epithelial cells.
    Figure Legend Snippet: The amount of urinary TECs can be measured using flow cytometry. (a) Gating strategy for selecting cytokeratin positive single cells. Staining of CD10, CD13, CD326, and (b) CD227 compared with isotype-controls in postmortem kidney tissue, (c) postmortem ureteral tissue, and (d) urine. Note CD326 but not CD227 positivity in ureteral epithelial cells. (e,f) Fluorescence microscopic image of (e) purified CK+DAPI+CD13+CD10+ cells, and (f) purified CK+DAPI+CD326+CD227+ cells (blue = DAPI, green = cytokeratin). (g–j) Electron microscopic images of purified cytokeratin+CD227+ cells using a cell sorter. (g and detail h) Apoptotic cell with characteristic marginalization of condensed chromatin (yellow arrows in h) and intact plasma membrane. (i and j) Necrotic cells with chromatin clumping (red arrow in j) and loss of plasma membrane integrity as distinguishing feature from apoptosis (yellow arrow in j). TECs, tubular epithelial cells.

    Techniques Used: Flow Cytometry, Staining, Fluorescence, Purification, Clinical Proteomics, Membrane



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    Image Search Results


    The amount of urinary TECs can be measured using flow cytometry. (a) Gating strategy for selecting cytokeratin positive single cells. Staining of CD10, CD13, CD326, and (b) CD227 compared with isotype-controls in postmortem kidney tissue, (c) postmortem ureteral tissue, and (d) urine. Note CD326 but not CD227 positivity in ureteral epithelial cells. (e,f) Fluorescence microscopic image of (e) purified CK+DAPI+CD13+CD10+ cells, and (f) purified CK+DAPI+CD326+CD227+ cells (blue = DAPI, green = cytokeratin). (g–j) Electron microscopic images of purified cytokeratin+CD227+ cells using a cell sorter. (g and detail h) Apoptotic cell with characteristic marginalization of condensed chromatin (yellow arrows in h) and intact plasma membrane. (i and j) Necrotic cells with chromatin clumping (red arrow in j) and loss of plasma membrane integrity as distinguishing feature from apoptosis (yellow arrow in j). TECs, tubular epithelial cells.

    Journal: Kidney International Reports

    Article Title: Flow-Cytometric Quantification of Urine Kidney Epithelial Cells Specifically Reflects Tubular Damage in Acute Kidney Diseases

    doi: 10.1016/j.ekir.2025.01.037

    Figure Lengend Snippet: The amount of urinary TECs can be measured using flow cytometry. (a) Gating strategy for selecting cytokeratin positive single cells. Staining of CD10, CD13, CD326, and (b) CD227 compared with isotype-controls in postmortem kidney tissue, (c) postmortem ureteral tissue, and (d) urine. Note CD326 but not CD227 positivity in ureteral epithelial cells. (e,f) Fluorescence microscopic image of (e) purified CK+DAPI+CD13+CD10+ cells, and (f) purified CK+DAPI+CD326+CD227+ cells (blue = DAPI, green = cytokeratin). (g–j) Electron microscopic images of purified cytokeratin+CD227+ cells using a cell sorter. (g and detail h) Apoptotic cell with characteristic marginalization of condensed chromatin (yellow arrows in h) and intact plasma membrane. (i and j) Necrotic cells with chromatin clumping (red arrow in j) and loss of plasma membrane integrity as distinguishing feature from apoptosis (yellow arrow in j). TECs, tubular epithelial cells.

    Article Snippet: The complete staining protocol included pan-cytokeratin-APC (clone: REA831; isotype: recombinant human IgG1) or, exclusively for fluorescence microscopy, pan-cytokeratin-FITC (clone: CK3-6H5; isotype: mouse IgG1), CD13-PE (clone: REA263; isotype: recombinant human IgG1), CD10-PE-Vio770 (clone: 97C5; isotype: mouse IgG1), CD326-PE (clone: HEA-125; isotype: mouse IgG1), and CD227-PE-Vio770 (clone: REA448; isotype: recombinant human IgG1) (all Miltenyi Biotec GmbH, Bergisch Gladbach, Germany).

    Techniques: Flow Cytometry, Staining, Fluorescence, Purification, Clinical Proteomics, Membrane

    Gating strategies for T cell subsets (A) and tubular epithelial cells (TEC) (B) . Isotype controls are displayed as blue, while full stains are represented in red. (C.1) Schematic overview of investigated subsets. Proximal TECs were defined CD10+ and CD13+, while distal TECs were characterized being CD227+ and CD326(EpCAM)+. (C.2) Maturation of naïve T cells into memory T cells. (D) Workflow for epigenetic analysis of urine samples. SSC, side scatter; FSC, forward scatter; TNV, naïve T cells; TEM, T effector memory cells; TCM, T central memory cells; TEMRA, T effector memory cells re-expressing CD45RA.

    Journal: Frontiers in Medicine

    Article Title: Urinary CD8+HLA-DR+ T Cell Abundance Non-invasively Predicts Kidney Transplant Rejection

    doi: 10.3389/fmed.2022.928516

    Figure Lengend Snippet: Gating strategies for T cell subsets (A) and tubular epithelial cells (TEC) (B) . Isotype controls are displayed as blue, while full stains are represented in red. (C.1) Schematic overview of investigated subsets. Proximal TECs were defined CD10+ and CD13+, while distal TECs were characterized being CD227+ and CD326(EpCAM)+. (C.2) Maturation of naïve T cells into memory T cells. (D) Workflow for epigenetic analysis of urine samples. SSC, side scatter; FSC, forward scatter; TNV, naïve T cells; TEM, T effector memory cells; TCM, T central memory cells; TEMRA, T effector memory cells re-expressing CD45RA.

    Article Snippet: The following antibodies were used: for T cells anti-CD3-APCeF780 (eBioscience, SK7, mo IgG1k), -CD4-PEVio770 (Miltenyi Biotec, REA623, REA) -CD8-APC (Biolegend, SK1, mo IgG1k) -CD45RO-PE (Biolegend, UCHL1, mo IgG1k2), -CD45-BUV805 (BD, 3D12, rat IgG1ak), -CCR7-BV421 (Biolegend, G043H7, mo IgG2ak), -HLA-DR-BUV395 (BD, G46-6, mo IgG2ak), -CD28-FITC (Biolegend, CD28.2, mo IgG1k) and for tubular epithelial cells anti-Cytokeratin-FITC (Miltenyi Biotec, CK3-6H5, mo IgG1k), -Vimentin-APC (Miltenyi Biotec, REA409, REA), -CD10-PeVio770 (Miltenyi Biotec, REA877, REA), -CD13-APCVio770 (Miltenyi Biotec, REA263, REA), -CD227-PE (Miltenyi Biotec, REA448, REA), -CD326-BV711 (Biolegend, 9C4, mo IgG2b).

    Techniques: Expressing